Porcine reproductive and respiratory syndrome virus vaccine

Rowland RR. Sow performance in an endemically porcine reproductive and respiratory syndrome PRRS -infected farm after sow vaccination with an attenuated PRRS vaccine. Vet Microbiol. Pejsak Z, Markowska-Daniel I. Randomised, placebo-controlled trial of a live vaccine against porcine reproductive and respiratory syndrome virus in sows on infected farms. Vet Rec. Impact of a modified-live porcine reproductive and respiratory syndrome virus vaccine intervention on a population of pigs infected with a heterologous isolate.

Effect of vaccination with a modified-live porcine reproductive and respiratory syndrome virus vaccine on dynamics of homologous viral infection in pigs. Am J Vet Res. Performance of fattening pigs in a farm infected with both porcine reproductive and respiratory syndrome PRRS virus and porcine circovirus type 2 following sow and piglet vaccination with an attenuated PRRS vaccine.

Assessment of the efficacy of commercial porcine reproductive and respiratory syndrome virus PRRSV vaccines based on measurement of serologic response, frequency of gamma-IFN-producing cells and virological parameters of protection upon challenge. Efficacy of combined porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae vaccination in piglets. Effect of vaccination on the potentiation of porcine reproductive and respiratory syndrome virus PRRSV -induced pneumonia by Mycoplasma hyopneumoniae.

The ever-expanding diversity of porcine reproductive and respiratory syndrome virus. Thanawongnuwech R, Suradhat S. The assessment of efficacy of porcine reproductive respiratory syndrome virus inactivated vaccine based on the viral quantity and inactivation methods. Virol J. Failure of an inactivated vaccine against porcine reproductive and respiratory syndrome to protect gilts against a heterologous challenge with PRRSV.

Novel strategies and approaches to develop the next generation of vaccines against porcine reproductive and respiratory syndrome virus PRRSV Virus Res. Charerntantanakul W. Adjuvants for porcine reproductive and respiratory syndrome virus vaccines. Construction and immunogenicity of DNA vaccines encoding fusion protein of murine complement C3d-p28 and GP5 gene of porcine reproductive and respiratory syndrome virus.

CD40 ligand expressed in adenovirus can improve the immunogenicity of the GP3 and GP5 of porcine reproductive and respiratory syndrome virus in swine. Strain specificity of the immune response of pigs following vaccination with various strains of porcine reproductive and respiratory syndrome virus. Comparative safety and efficacy of attenuated single-strain and multi-strain vaccines for porcine reproductive and respiratory syndrome.

Pirzadeh B, Dea S. Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus. J Gen Virol. Immunisation of pigs with a major envelope protein sub-unit vaccine against porcine reproductive and respiratory syndrome virus PRRSV results in enhanced clinical disease following experimental challenge.

Vet J. Construction and characterization of a recombinant canine adenovirus expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus.

J Vet Med Sci. Immune responses in pigs induced by recombinant canine adenovirus 2 expressing the glycoprotein 5 of porcine reproductive and respiratory syndrome virus. Vet Res Commun. Immunogenicity and protective efficacy of recombinant pseudorabies virus expressing the two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus. Protective immunity induced by a recombinant pseudorabies virus expressing the GP5 of porcine reproductive and respiratory syndrome virus in piglets.

Co-expressing GP5 and M proteins under different promoters in recombinant modified vaccinia virus ankara rMVA -based vaccine vector enhanced the humoral and cellular immune responses of porcine reproductive and respiratory syndrome virus PRRSV Virus Genes.

Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus. Virus Genes. Chen X, Liu J. Generation and immunogenicity of transgenic potato expressing the GP5 protein of porcine reproductive and respiratory syndrome virus. J Virol Methods. Immunogenicity of recombinant GP5 protein of porcine reproductive and respiratory syndrome virus expressed in tobacco plant.

Oral immunization induces local and distant mucosal immunity in swine. What role does the route of immunization play in the generation of protective immunity against mucosal pathogens?

J Immunol. Mucosal adjuvant activity of cholera toxin requires Th17 cells and protects against inhalation anthrax. Development of a porcine reproductive and respiratory syndrome virus differentiable DIVA strain through deletion of specific immunodominant epitopes. DNA Cell Biol. Fang Y, Snijder EJ. The PRRSV replicase: exploring the multifunctionality of an intriguing set of nonstructural proteins. Mol Immunol. Dokland T. Effects of a modified-live virus vaccine against porcine reproductive and respiratory syndrome in boars.

Ansari I. Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies. Immune evasion of porcine reproductive and respiratory syndrome virus through glycan shielding involves both glycoprotein 5 as well as glycoprotein 3.

Tian D. Arterivirus minor envelope proteins are a major determinant of viral tropism in cell culture. Zhang H. Kim W.

Different biological characteristics of wild-type porcine reproductive and respiratory syndrome viruses and vaccine viruses and identification of the corresponding genetic determinants. Xie J. Song J. Safety and protective efficacy of porcine reproductive and respiratory syndrome recombinant virus vaccines in young pigs.

Molecular assessment of the role of envelope-associated structural proteins in cross neutralization among different PRRS viruses. Virus Genes. Kimpston-Burkgren K. Relative contribution of porcine reproductive and respiratory syndrome virus open reading frames 2—4 to the induction of protective immunity.

Fan B. The amino acid residues at and in GP5 of porcine reproductive and respiratory syndrome virus regulate viral neutralization susceptibility to the porcine serum neutralizing antibody. Trible B. A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity. Rappe J. Heterogeneous antigenic properties of the porcine reproductive and respiratory syndrome virus nucleocapsid.

Kwon B. Identification of virulence determinants of porcine reproductive and respiratory syndrome virus through construction of chimeric clones. Wang Y.

Attenuation of porcine reproductive and respiratory syndrome virus strain MN using chimeric construction with vaccine sequence. Nsp9 and Nsp10 contribute to the fatal virulence of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China. PLoS Pathog. Zhao K. Beura L. Identification of amino acid residues important for anti-IFN activity of porcine reproductive and respiratory syndrome virus non-structural protein 1.

Han M. Nuclear imprisonment of host cellular mRNA by nsp1beta protein of porcine reproductive and respiratory syndrome virus. Targeted mutations in a highly conserved motif of the nsp1beta protein impair the interferon antagonizing activity of porcine reproductive and respiratory syndrome virus.

Utilizing host endogenous microRNAs to negatively regulate the replication of porcine reproductive and respiratory syndrome virus in MARC cells. Liu X. Effect of amino acids residues — and in Nsp2 of representative porcine reproductive and respiratory syndrome virus strains on inflammatory response in vitro. The middle half genome of interferon-inducing porcine reproductive and respiratory syndrome virus strain A2MC2 is essential for interferon induction.

Sun H. Identification of viral genes associated with the interferon-inducing phenotype of a synthetic porcine reproductive and respiratory syndrome virus strain.

The N-N non-covalent domain of the nucleocapsid protein of type 2 porcine reproductive and respiratory syndrome virus enhances induction of IL expression. Subramaniam S. Amino acid residues in the non-structural protein 1 of porcine reproductive and respiratory syndrome virus involved in down-regulation of TNF-alpha expression in vitro and attenuation in vivo. Attenuation of porcine reproductive and respiratory syndrome virus by molecular breeding of virus envelope genes from genetically divergent strains.

Gao L. Computer-aided codon-pairs deoptimization of the major envelope GP5 gene attenuates porcine reproductive and respiratory syndrome virus. Park C. Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs.

Zhou L. Cao Q. Immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine GM-CSF. Sang Y.

Replication-competent recombinant porcine reproductive and respiratory syndrome PRRS viruses expressing indicator proteins and antiviral cytokines.

De Lima M. Development of a porcine reproductive and respiratory syndrome virus differentiable DIVA strain through deletion of specific immunodominant epitopes. Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: Implications for future recombinant marker vaccine development. Pt 12 J. Characterization of a serologic marker candidate for development of a live-attenuated DIVA vaccine against porcine reproductive and respiratory syndrome virus.

Recombinant PRRSV expressing porcine circovirus sequence reveals novel aspect of transcriptional control of porcine arterivirus. Porcine reproductive and respiratory syndrome virus as a vector: Immunogenicity of green fluorescent protein and porcine circovirus type 2 capsid expressed from dedicated subgenomic RNAs.

Protective efficacy of a virus-vectored multi-component vaccine against porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and swine influenza virus. Kappes M. Highly divergent strains of porcine reproductive and respiratory syndrome virus incorporate multiple isoforms of nonstructural protein 2 into virions.

Guo R. Suhardiman M. Generation of porcine reproductive and respiratory syndrome virus by in vitro assembly of viral genomic cDNA fragments. Meulenberg J. Infectious transcripts from cloned genome-length cDNA of porcine reproductive and respiratory syndrome virus. Huang Y. Identification and characterization of a porcine monocytic cell line supporting porcine reproductive and respiratory syndrome virus PRRSV replication and progeny virion production by using an improved DNA-launched PRRSV reverse genetics system.

A DNA-launched reverse genetics system for porcine reproductive and respiratory syndrome virus reveals that homodimerization of the nucleocapsid protein is essential for virus infectivity. Establishment of a DNA-launched infectious clone for a highly pneumovirulent strain of type 2 porcine reproductive and respiratory syndrome virus: Identification and in vitro and in vivo characterization of a large spontaneous deletion in the nsp2 region. An infectious cDNA clone of porcine reproductive and respiratory syndrome virus.

Truong H. A highly pathogenic porcine reproductive and respiratory syndrome virus generated from an infectious cDNA clone retains the in vivo virulence and transmissibility properties of the parental virus.

A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: Expression of green fluorescent protein in the Nsp2 region. Nielsen H. Generation of an infectious clone of VR, a highly virulent North American-type isolate of porcine reproductive and respiratory syndrome virus.

Goldberg T. Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection. Boyer J. Wang T. Microbes Infect.

A kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Johnson C. Novel structural protein in porcine reproductive and respiratory syndrome virus encoded by an alternative ORF5 present in all arteriviruses.

Doan D. Structure of the nucleocapsid protein of porcine reproductive and respiratory syndrome virus. Song B. Packaging of porcine reproductive and respiratory syndrome virus replicon RNA by a stable cell line expressing its nucleocapsid protein. Zhang R. Disulfide linkages mediating nucleocapsid protein dimerization are not required for porcine arterivirus infectivity. Rowland R. The localization of porcine reproductive and respiratory syndrome virus nucleocapsid protein to the nucleolus of infected cells and identification of a potential nucleolar localization signal sequence.

Kim D. Insertion and deletion in a non-essential region of the nonstructural protein 2 nsp2 of porcine reproductive and respiratory syndrome PRRS virus: Effects on virulence and immunogenicity.

Chen Z. Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication, but play an important role in modulation of the host immune response. Ran Z. Recovery of viable porcine reproductive and respiratory syndrome virus from an infectious clone containing a partial deletion within the Nsp2-encoding region.

Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain HuN4-F of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc cell. Stable expression of foreign gene in nonessential region of nonstructural protein 2 nsp2 of porcine reproductive and respiratory syndrome virus: Applications for marker vaccine design.

Vigerust D. Virus glycosylation: Role in virulence and immune interactions. Trends Microbiol. Dea S. Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome PRRS virus: Comparison of the North American and European isolates. Mardassi H. Molecular analysis of the ORFs 3 to 7 of porcine reproductive and respiratory syndrome virus, Quebec reference strain. The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus.

Faaberg K. Neutralizing antibody responses of pigs infected with natural GP5 N-glycan mutants of porcine reproductive and respiratory syndrome virus. Helle F. The hepatitis C virus glycan shield and evasion of the humoral immune response. Lee J. Protective humoral immune response induced by an inactivated porcine reproductive and respiratory syndrome virus expressing the hypo-glycosylated glycoprotein 5.

Van Breedam W. Porcine reproductive and respiratory syndrome virus entry into the porcine macrophage. Dobbe J. Construction of chimeric arteriviruses reveals that the ectodomain of the major glycoprotein is not the main determinant of equine arteritis virus tropism in cell culture.

Chimeric viruses containing the N-terminal ectodomains of GP5 and M proteins of porcine reproductive and respiratory syndrome virus do not change the cellular tropism of equine arteritis virus. Kim H. Enhanced replication of porcine reproductive and respiratory syndrome PRRS virus in a homogeneous subpopulation of MA cell line. Murtaugh M. Immunological solutions for treatment and prevention of porcine reproductive and respiratory syndrome PRRS Vaccine.

Ostrowski M. Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. Calvert J. CD expression confers susceptibility to porcine reproductive and respiratory syndrome viruses. Tian K. The amino-acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence. The viral innate immune antagonism and an alternative vaccine design for PRRS virus.

Yang L. The cysteine protease domain of porcine reproductive and respiratory syndrome virus nonstructural protein 2 possesses deubiquitinating and interferon antagonism functions. Nonstructural protein 2 of porcine reproductive and respiratory syndrome virus inhibits the antiviral function of interferon-stimulated gene Wang X. Development of a differentiable virus via a spontaneous deletion in the nsp2 region associated with cell adaptation of porcine reproductive and respiratory syndrome virus.

Porcine reproductive and respiratory syndrome virus nonstructural protein 1beta modulates host innate immune response by antagonizing IRF3 activation. Sun Y. Nan Y. Induction of type I interferons by a novel porcine reproductive and respiratory syndrome virus isolate. Development of a broadly protective modified-live virus vaccine candidate against porcine reproductive and respiratory syndrome virus. Abstract Current vaccines do not provide sufficient levels of protection against divergent porcine reproductive and respiratory syndrome virus PRRSV strains circulating in the field, mainly due to the substantial variation of the viral genome.

Publication types Research Support, U. Gov't, Non-P.