Olympus confocal software manual

This interface can be utilized to change from laser scanning to widefield imaging modes and to adjust the microscope focus and filter wheel positions on motorized units. The SU button activates a setup window illustrating the current scanning unit configuration. This menu can be employed to toggle laser systems into standby mode or activate Auto Standby and to change the pinhole aperture size, dichromatic mirrors, and filter wheels in the unit.

Routine x-y-z scanning operations and time-lapse observations both produce multiple images, which can be displayed in sequence simply by clicking the sequential mode button. Channel selection and image zooming are also available on the same menu.

Simple performance of useful functions, such as multi-channel image overlay and channel selection options, are accessed with a four-button menu. In order to access frequently used commands, the right mouse button can be clicked in the display area to launch a pop-up menu.

A series of frequently used software commands may be stored as a macro command file so that a particular imaging sequence or analysis strategy can be easily repeated. Online help enables the user to obtain reference information about individual functions and procedures while operating the applications. Support documents are easy to understand and are often accompanied by animated icons, such as a light path exchange lever indicated by a flashing red light when setting up the microscope.

The most frequently used functions can be accessed via buttons on the horizontal toolbar located at the bottom left of the interface window. Icons simplify function identification, such as a color bar for pseudo-color processing through look-up table editing and a pencil tool for inserting comments on measurements and images.

Observation of a specimen over an extended period of time or with sectional imaging results in the generation of a significant number of images. The tiling software function provides versatile viewing options, including simultaneous multiple or multi-channel image display. The background gradient often observed in differential interference contrast DIC images can be compensated with a single mouse click without the necessity of a reference image.

This function maintains every detail of the image. The ability to preset storage and settings values on a file containing all of the relevant image acquisition conditions enables immediate, one-touch recall. Adding new conditions or altering existing ones is quick and easy. Scan mode selection and other basic operations can be performed by clicking on simple and intuitive key combinations on the keyboard. Software Features Tab-Style Monitor Display One-touch tab menu selection makes it easy to handle a multiple range of functions.

Follow the instructions detailed in this section to successfully download selected software. Built around the Install Shield programming interface, the Olympus MIC-D digital microscope screen savers and support software are very simple to install. First double-click on the appropriate icon, and then follow the instructions presented in the subsequent dialog boxes. The MIC-D screen savers each have12 user-configurable image-switching modules that allow a variety of transitions between images.

This feature enables the user to customize the screen saver display and activity to suit personal tastes. Mortimer Abramowitz - Olympus America, Inc. John C. Long , Kirill I. Tchourioukanov , and Michael W. Background Subtraction Toolkit Download The Olympus Microscopy Resource Center MIC-D digital microscope Background Subtraction Toolkit is a stand-alone Java application program designed for the Windows operating system, which can be utilized to produce uniform backgrounds for digital images captured with this unique inverted optical microscope.

The Olympus FluoView TM FV is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens.

While one laser stimulates, the second laser simultaneously provides high-resolution imaging. Features and Benefits - Among the advanced features of the FV is the simultaneous application of two laser scanners SIM scanner to enable synchronization of specimen excitation and observation.

Highly efficient ion deposition interference filters increase the instrument's sensitivity, while high-speed galvanometers and spectroscopic detection combine to yield precision scans with 2-nanometer wavelength resolution.

FV Scanning Unit - The newly developed FV scanning units offer a high-sensitivity detection system coupled to high-speed scanning and laser feedback to enable the capture of high-quality images of living organisms undergoing rapid change.

Images may be scanned in any pixel array size up to x , with each channel digitized to gray levels bit resolution , permitting observation of fine image detail.